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Sains Malaysiana 53(11)(2024): 3663-3672
http://doi.org/10.17576/jsm-2024-5311-10
Intestinal
Piezo1 Promotes Neuroinflammation to Facilitate Oligodendrocyte Ferroptosis
Post-Traumatic Brain Injury
(Usus Piezo1 Menggalakkan Keradangan Neuro untuk Memudahkan Oligodendrocyte Ferroptosis Kecederaan Otak Pasca Traumatik)
DING ZHI JUN1, WU LIANG1,
CHEN ZHENG XIONG1, ZENG RUI MAO1, SHANGYUAN WANG2 & TANG MING ZHANG2,*
1Department
of Neurosurgery Department, Ningde Municipal Hospital
of Ningde Normal University, Fujian, China
2Department
of Emergency, Xinhua Hospital Affiliated to Shanghai Jiao Tong University
School of Medicine, Shanghai, China
Received
20 August 2024/Accepted 23 September 2024
Abstract
The
coexistence of traumatic brain injury (TBI) and cognitive impairment is
increasingly common in clinical practice, but current research on identifying
biomarkers for such conditions and precise intervention points is insufficient.
This study utilized bioinformatics analysis with paired samples to explore
potential causal links between inflammatory factors and TBI and cognitive
impairment. By constructing a TBI model with Piezo1 gene knockout, we assessed
the activation status of microglia in the brain, the differentiation process of
oligodendrocyte precursor cells. The study identified a series of inflammatory
factors significantly associated with TBI, including C-C motif chemokine 19,
C-X-C motif chemokine 5, and interleukin-5. Bioinformatics analysis showed
increased expression of CXCL10, CCL2, GNA15, NFKB1, and the top 5 key nodes
were identified using the Cytohubba plugin. The
experimental results indicated that the knockout of the Piezo1 gene
significantly reduced the infiltration of microglia and neuroinflammation in
the brain.
Keywords: Bioinformatics; CXCL10; neuroinflammation; traumatic brain injury
Abstrak
Kewujudan kecederaan otak traumatis (TBI) dan gangguan kognitif semakin menjadi kebiasaan dalam amalan klinikal, tetapi penyelidikan semasa mengenai pengenalpastian penanda biologi untuk keadaan sedemikian dan titik intervensi yang tepat adalah tidak mencukupi.
Kajian ini menggunakan analisis bioinformatik dan randomisasi Mendelian (MR) dengan sampel bersama untuk meneroka hubungan kausal berpotensi antara faktor inflamasi dengan TBI dan gangguan kognitif. Dengan membina model TBI dengan penutupan gen Piezo1, kami menilai status pengaktifan mikroglia dalam otak, proses pembezaan sel-sel pendahulu oligodendrosit. Kajian mengenal pasti siri faktor inflamasi yang signifikan berkaitan dengan TBI, termasuk kemokina motif C-C 19, kemokina motif C-X-C 5 dan interleukin-5. Analisis bioinformatik menunjukkan peningkatan ekspresi CXCL10,
CCL2, GNA15, NFKB1 dan 5 nod kunci teratas dikenal pasti menggunakan plugin Cytohubba. Keputusan uji kaji menunjukkan bahawa penutupan gen Piezo1 secara signifikan mengurangkan infiltrasi mikroglia dalam otak dan melindungi pembezaan oligodendrosit.
Kata kunci: Bioinformatik;
CXCL10; kecederaan otak traumatis; keradangan neuro
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*Corresponding
author; email: 350571178@qq.com
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